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Wednesday, July 22, 2020 | History

2 edition of -tubulin gene family of Chinese hamster ovary cells. found in the catalog.

-tubulin gene family of Chinese hamster ovary cells.

Elizabeth Margaret Elliott

-tubulin gene family of Chinese hamster ovary cells.

by Elizabeth Margaret Elliott

  • 120 Want to read
  • 16 Currently reading

Published .
Written in English


The Physical Object
Pagination[133] leaves
Number of Pages133
ID Numbers
Open LibraryOL14758759M

The characteristic sequence of β-interferon matrix attachment regions (MARs) can mediate transgene expression via episomal vectors in Chinese hamster ovar. Gene. The human ERCC4 gene can correct the DNA repair defect in specific ultraviolet light (UV)-sensitive mutant cell lines derived from Chinese hamster ovary cells. Multiple independent complementation groups of Chinese hamster ovary (CHO) cells have been isolated, and this gene restored UV resistance to cells of complementation group 4. Reflecting this cross-species genetic .

The indicated target cells were plated at 10 5 per 6-cm dish on day 1, infected with an amphotropic-pseudotype vector carrying the neo gene on day 2 in the presence of culture medium containing 5% FBS (no additional serum), 5% FBS plus 5% Chinese hamster serum, 5% FBS plus % Syrian hamster serum, or 5% FBS plus 25% additional FBS, and G   Chinese hamster ovary-K1 cells were transiently transfected with either the pCMV6 or pCMV6-FLAG-mWS plasmid and then incubated with primary antibodies (anti-calnexin carboxyl terminus rabbit polyclonal antibody (Stressgen, San Diego) at a dilution and/or α-FLAG M2 monoclonal antibody (Sigma) at a dilution). Cells were incubated for.

Quiescent Chinese hamster ovary cells, as well as three other types of quiescent cells, synthesize histone at a reduced but significant rate. The variant patterns of the histone synthesis in quiescent, S-phase and G1-phase and G2-phase cells all differed from each other. H was the only H3 variant synthesized in quiescent and in G1 and G2 cells. The main purpose of transfection is to study the function of genes or gene products, by enhancing or inhibiting specific gene expression in cells, and to produce recombinant proteins in mammalian cells [3]. Examples are: gene therapy delivering a gene of interest into cells to cure a disease or improve symptoms; induced pluripotent stem cell.


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-tubulin gene family of Chinese hamster ovary cells by Elizabeth Margaret Elliott Download PDF EPUB FB2

Chinese hamster ovary (CHO) cells are the most common cells applied in the commercial production of biopharmaceuticals. This cell line isolated in the s gave rise to a range of genetically different progeny, such as K1- DukX B, DGcell lines and others which differ in protein product quality and achievable by: ISBN: OCLC Number: Description: xxvii, pages: illustrations ; 24 cm: Contents: Cytogenetic consequences of gene amplification: HSRs and DMs in antifolate-resistant and multidrug-resistant Chinese hamster and mouse cells and in human neuroblastoma cells with amplified N-myc protooncogenes --Organization and structure of.

It is produced primarily in the kidney and is an essential hormone for initiating and regulating the production of red blood cells. Recombinant erythropoietin (r-EPO) has been produced by the introduction of the human erythropoietin gene into Chinese hamster ovary cells [65, 66].

The production of anti-HER2 -tubulin gene family of Chinese hamster ovary cells. book from the FUT8 gene knockout CHO-S cells in the bioreactor increased by 37% compared to the shake flask system. we engineered Chinese hamster ovary cells.

The human excision repair gene ERCC-1 was cloned after DNA mediated gene transfer to the CHO mutant B, which is sensitive to ultraviolet light and mitomycin-C.

We describe the cloning and sequence analysis of the ERCC-1 cDNA and partial characterization of the gene. ERCC-1 has a size of 15 kb and is located on human chromosome The ERCC-1 precursor RNA is subject to alternative Cited by: A Chinese Hamster Ovary (CHO) cell line containing the human gamma interferon (IFN-∝) gene co-amplified with the gene for dihydrofolate reductase (DHFR), was continuously maintained for days in serum-free medium with μM methotrexate.

During this time the IFN-∝ titre fell by 70% due to a reduction in the specific IFN production rate. CHO cells had been used for many years in laboratory work beginning in for typing pneumococci.

Their use declined after domestication problems led to hereditary diseases due to imbreeding. Then inDr. Theodore T. Puck from the University of Colorado’s Department of Medicine first established ovary cells in culture plates and found.

Chinese Hamster Ovary cells (CHO-K1) were used to produce a recombinant mucin-type fusion protein with tailored glycosylation by expressing P-selectin glycoprotein ligand 1/mouse immunoglobulin. Chinese hamster ovary cells, a type of mammalian cells often used in biotech manufacturing, are generally used to produce a humanlike version of EPO for pharmaceutical use.

• EPO must be produced in mammalian cells because microbes like bacteria and yeast don’t have the cellular machinery to stick the critical sugar chains onto the protein. Reynolds JE, Yang T, Qian L, Jenkinson JD, Zhou P, Eastman A, Craig RW () Mcl-1, a member of the Bcl-2 family, delays apoptosis induced by c-Myc overexpression in Chinese hamster ovary cells.

Cancer Res – PubMed Google Scholar. Chinese hamster ovary (CHO) cells are a source of multi-ton quantities of protein pharmaceuticals. They are, however, immortalized cells, characterized by a high degree of genetic and phenotypic diversity. As is known for any biological system, this diversity is enhanced by selective forces when laboratories (no sharing of gene pools) grow cells under (diverse) conditions that are practical.

Micrococcus luteus UV-Endonuclease-Sensitive Sites in Far- and Near-UV-Irradiated Chinese Hamster Ovary Cells 6. Repair of Alkylated DNA in Chinese Hamster Cells Measured by Loss of Enzyme-Sensitive Sites in Isolated DNA 7.

Serologic Assay of DNA Base Damage 8. Non-dimer UV Damage in DNA and Poly d(A-T) 9. This phenomenon was observed in several cell types, including Chinese hamster ovary cells as well as the AT cell line.

The occurrence of gene amplification in drug-resistant cells was observed not only with DHFR but also with the carbamoyl-synthetase 2 aspartate transcarbamylase and dihydroorotase (CAD) gene. In this updated and expanded second edition of an established classic, the editors have added critical reviews to a fresh collection of cutting-edge protocols for gene expression in bacteria, fungi, plants, plant cells, animals, and animal cells.

The review articles survey new directions in recombinant gene expression research, technique, and application, and point the way to using recombinant 2/5(1). Chinese hamster V79 cells and their BRCA2 deficient mutant V-C8, and V-C8 with gene complemented cells were tested against epigallocatechin gallate and theaflavin.

In addition, Chinese hamster ovary (CHO) wild-type cells and rad51D mutant 51D1 cells were used to further investigate the synthetic lethality of these molecules.

Isolation of a chinese hamster ovary (CHO) cDNA encoding phosphatidylglycerophosphate (PGP) synthase, expression of which corrects the mitochondrial abnormalities of a PGP synthase-defective mutant of CHO-K1 cells. Biol. ChemCrossref, Medline, Google Scholar; Kim M.

Y., Linardic C., Obeid L., Hannun Y. Mohan, C., and Lee, G. () Effect of inducible co-overexpression of protein disulfide isomerase and endoplasmic reticulum oxidoreductase on the specific antibody productivity of recombinant Chinese hamster ovary cells, Biotechnol Bioeng.

– @article{osti_, title = {Effects of preventing O-glycosylation on the secretion of human chorionic gonadotropin in Chinese hamster ovary cells}, author = {Matzuk, M M and Krieger, M and Corless, C L and Boime, I}, abstractNote = {Human chorionic gonadotropin (hCG) is a member of a family of heterodimeric glycoprotein hormones that have a alpha.

subunit but differ in their. How a Chinese hamster came to dominate production of biopharmaceuticals. Little did you know, but a single hamster has been a force of innovation and massive biomedical production.

Scientists devote entire careers to so called CHO cells from this Chinese hamster’s ovary. Many recombinant glycoproteins have been expressed in Chinese hamster ovary (CHO) cells and analysis of their carbohydrates has shown that, in common with human cells, CHO cells contain a large repertoire of glycosylating enzymes and are therefore able to process a wide variety of glycans.

In this updated and expanded second edition of an established classic, the editors have added critical reviews to a fresh collection of cutting-edge protocols for gene expression in bacteria, fungi, plants, plant cells, animals, and animal cells.

Horizon will use Mammoth's platform to generate a new line of Chinese hamster ovary cells — a common source for making therapeutic proteins — to make antibodies and other drugs.Neurokinins are a family of neuropeptides with widespread distribution mediating a broad spectrum of physiological actions through three distinct receptor subtypes: NK-1, NK-2, and NK We investi.